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KMID : 1098420020100040243
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Korean Journal of Medicinal Crop Science
2002 Volume.10 No. 4 p.243 ~ p.248
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Optimization of Major Culture Elements on Growth and Shikonin Production in the Lithospermum erythrorhizon Hairy Root Culture
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Hwang, Ok Jin
Kim, Yu Jeong/Sung, Nak Sul/Ahn, Jun Cheul/Kim, Sik Eung/Hwang, Baik
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Abstract
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The effects of basal media, carbon, nitrogen, phposphate and some major macro elements on growth and shikonin production in Lithospermum erythrorhizon hairy root culture were studied. Among examined media, growth of hairy root cultured in B5 liquid medium was rapid, whereas shikonin production was high in MS liquid medium. Under B5 basal medium was rapid, sucrose concentration for optimal growth and shikonin production was 9% and 4% respectively. The growth and shikonin production on pH changes in B5 medium resulted little effect in pH 5.8 to pH 8.8 ranges, whereas growth was decreased dramatically in both above 8.8 and under 5.8. Nitrogen source and concentration effected on the growth and shikonin production. The highest growth rate was in B5 medium (50 mM KNO_3, and 10 mM NaH_2PO_4), whereas the highest shikonin production was in the condition supplemented with 5 mM KNO_3 and 10 mM NaH_2PO_4.
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